The WEISS Lab


Pathophysiology of Ion Channels

Selective inhibition of neuronal Cav3.3 T-type calcium channels by TAT-based channel peptide


Journal article


Leos Cmarko, N. Weiss
Molecular Brain, 2020

Semantic Scholar DOI PubMedCentral PubMed
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APA
Cmarko, L., & Weiss, N. (2020). Selective inhibition of neuronal Cav3.3 T-type calcium channels by TAT-based channel peptide. Molecular Brain.

Chicago/Turabian
Cmarko, Leos, and N. Weiss. “Selective Inhibition of Neuronal Cav3.3 T-Type Calcium Channels by TAT-Based Channel Peptide.” Molecular Brain (2020).

MLA
Cmarko, Leos, and N. Weiss. “Selective Inhibition of Neuronal Cav3.3 T-Type Calcium Channels by TAT-Based Channel Peptide.” Molecular Brain, 2020.


Abstract

Low-voltage-activated Cav3 calcium channels (T-type) play an essential role in the functioning of the nervous system where they support oscillatory activities that relie on several channel molecular determinants that shape their unique gating properties. In a previous study, we documented the important role of the carboxy proximal region in the functioning of Cav3.3 channels. Here, we explore the ability of a TAT-based cell penetrating peptide containing this carboxy proximal region (TAT-C3P) to modulate the activity of Cav3 channels. We show that chronic application of TAT-C3P on tsA-201 cells expressing Cav3 channels selectively inhibits Cav3.3 channels without affecting Cav3.1 and Cav3.2 channels. Therefore, the TAT-C3P peptide described in this study represents a new tool to address the specific physiological role of Cav3.3 channels, and to potentially enhance our understanding of Cav3.3 in disease.


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